[00:00:00] Carl Lanore: [00:00:00] welcome back to another episode of superhuman radio. Today is September 17th, 2020. And we have a very interesting show for you today. Um, we're going to be talking about how we can use our sweat to evaluate a certain metabolites. You know, we know about blood and we know about saliva. Uh, sweat is another bodily fluid, but in the past it's been very difficult to use a sweat.

[00:00:26] Uh, to do anything, but some recent research shows that maybe they were looking for the wrong things in the past. Uh, before we do that, we have to thank our title sponsor, legendary foods. The website is legendary.com. If you use the code SHR 10, you will save 10% off of all of your purchases there.

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[00:01:33] Dr. Sira Karvinen, PhD: [00:01:33] Yes.

[00:01:34] Good,

[00:01:34] Carl Lanore: [00:01:34] good. Okay. And I'm going to attempt to, to, uh, mentioned the, a university that you're affiliated with. So you are in the department of gerontology research and faculty of sport and health sciences at the university of Java Scala in Finland. Yes. Is that how you pronounce it? Java Scala. Okay, thank you.

[00:01:58] Thank you for correcting me. Okay. [00:02:00] No problem. Welcome to the show. Welcome to the show. So, um, this is interesting exercise, induces secretion of biomarkers into sweat. What research preceded this, that, uh, your study was required to elucidate these new details?

[00:02:18] Dr. Sira Karvinen, PhD: [00:02:18] Well, this is actually quite new deal for us to be honest.

[00:02:23] So we started off with thought there is actually a need for, um, exercise biomarkers that could be collecting  since we're a sport faculty. So know that as you were warm, I mean, um, exercise intensity that can only be done by a blog, and then you measure normally lactate levels. And so we saw. Well, it's all normal bipolar exercise.

[00:02:55] So if it would be possible, do you, that's what I have markers or [00:03:00] tell us about how our body opening and fall, how it tends to exercise is for our body would be great.

[00:03:09] Carl Lanore: [00:03:09] So what specific biomarkers were of interest to your group?

[00:03:14] Dr. Sira Karvinen, PhD: [00:03:14] Uh, to our group, we decided to take my priorities. Um, since, um, we, we have studied those in, in our research for previously, and also there's a lot of literature from, um, serum or plasma that my car and a scan.

[00:03:34] You have a nice response to, to endurance exercise for instance. So it was already known that in blog, there's a response. So we kind of wanted to take a look at sweat that could we actually find something similar from there.

[00:03:50] Carl Lanore: [00:03:50] And so what, when, when you exercise intensely, what kind of changes do you see in micro RNA that, that you would like, that you were [00:04:00] looking for in sweat?

[00:04:00] Yeah. Yeah.

[00:04:03] Dr. Sira Karvinen, PhD: [00:04:03] Yeah. Well, we went. With the micro RNs that we have seen in previous literature, givers, phones in CRM or in plasma. So we chose six different micro RNs that are normally, they respond halt to chronic and acute exercise. And now in our study center. So we had acute exercise responses that we were looking at.

[00:04:27] And what we found from sweats was the micro RNA. 20th six was a very interesting, so we kind of gave a step wise increase essay, exercise, intensity priest. So that was our main finding.

[00:04:42] Carl Lanore: [00:04:42] And so the, uh, I mean, sweat has been used in the past for certain things. But it was not thought that you could see these types of results in sweat, because there just isn't enough of this in sweat.

[00:04:56] Is that the problem there's not enough concentration. You can't [00:05:00] gather enough sweat to actually look for metabolites, uh, effected by exercise.

[00:05:07] Dr. Sira Karvinen, PhD: [00:05:07] Yes. That is the main problem. So there's very few biomolecular, you know, her milliliter of sweat, for instance, right. Oh, incredulously here. True. That has looked at for instances, swept metal halides or different.

[00:05:22] They are Meeks. Um, they need tongue soft sweat. Basically there has to be 10 often leaders of sweats for you to be able to, you know, detect anything in a vote this way and offers. If you think of, um, having a non invasive, um, When he train system, no one can produce tens of MLS, sweat to the monitor and stuff.

[00:05:47] So what we wanted to do is to check the micro RNA from a relatively small volume, this sweat. So we have only one ML, and now we need to bear in mind that, um, [00:06:00] methods, how we studied with this. A lot different than what you would you for is this for, for proteins you use mass spec or metabolites is when we have microgrids, when those are our target, then we use the PCR, which is basically multiplying.

[00:06:18] So in a way it's both problems, sort of just, the whole idea is to move it by that

[00:06:24] Carl Lanore: [00:06:24] signal. Interesting. So you discovered these micro RNA presence in, uh, the sweat of individuals who exercise intensely. In fact, uh, I think one group actually did high intensity training on for cardio, right? Is that what they were doing?

[00:06:45] Dr. Sira Karvinen, PhD: [00:06:45] Yeah, they were doing actually, um, three different protocols. So the same subject stated first, the VO two max tests. And according to that, we then determined the Arabic trust hall [00:07:00] and the Arabic trust halls of these people. And then we also made them do a exercise 30 minutes roughly on Arabic trust hold like 5% through all first week from adult.

[00:07:13] Right at. At the trust hold and then also facing would exercise as Arabic

[00:07:18] Carl Lanore: [00:07:18] trust hold. And you also, I'm sorry, go ahead. I'm sorry. I didn't mean to interrupt you.

[00:07:23] Dr. Sira Karvinen, PhD: [00:07:23] Yeah. Okay. So they did these three different protocols and then we had also, uh,

[00:07:28] Carl Lanore: [00:07:28] that's the one I'm interested in the song. So the sauna was the control because basically you're.

[00:07:36] You're you are sweating, but you're not exercising. So theoretically you would be able to differentiate between sauna, sweat, and sweat produced by exercise because of the presence of this, uh, MRI, this micro RNA, right? Yes. And did you find that, did you find that the people who just sat in this, of course, because there's emerging evidence right now that [00:08:00] sauna.

[00:08:01] Which you're in Finland, right? I mean, sauna is big in Finland and I love sauna. I have a sauna at home for that reason. Um, but, but there's a, some emerging opinion right now that sauna conveys some of the same benefits as exercise. They're now saying it's an exercise mimetic. However, there's a big difference between walking and sweating.

[00:08:29] And high intensity training and sweating. So did you see any presence of the micro RNA and sauna that would indicate there was some mild effect that would be, could be compared to exercise in sauna?

[00:08:46] Dr. Sira Karvinen, PhD: [00:08:46] That's a good question. And it's kind of good critique also for the setup because it is. In almost impossible to have a person sweating without, [00:09:00] you know, having anything to do it.

[00:09:03] So having a control for a sweat sample with non-exercise is really difficult. And we use the test as a control here, so we could detect the same micro RNs in the sweat, also in the Sala sample. But then again, um, the levels are much higher. When it's a search and exercise test, but of course I cannot say for a hundred percent sure that how would it be if we could induce the sweating without even the temperature increase?

[00:09:37] Carl Lanore: [00:09:37] Yeah. I mean the only way to increase. Uh, to produce a sweat without exercise would be, if someone had, for instance, a fever, uh, if they were sick, they may sweat. Um, and it's hard to make people sick in a lab so that you can test their sweat, obviously. So the saw, I could see why you use the sauna. What exactly are extracellular [00:10:00] vesicles.

[00:10:03] Um,

[00:10:04] Dr. Sira Karvinen, PhD: [00:10:04] where they're membrane bound, vesicles that are secreted from various kind of cells. Um, they are kind of the text messages of self. So to say, so they transferred information from cell to cell and ulcer from PQ to tissue. So they're kind of transport vehicles or for different kind of biomolecules.

[00:10:28] Carl Lanore: [00:10:28] So could you compare them to like liposomes liposomes or.

[00:10:32] Are fat bubbles, if you will, that encapsulate things, would it be kind of like that?

[00:10:40] Dr. Sira Karvinen, PhD: [00:10:40] If it does have the only bit membrane? Yes. It also has proteins in there. So I don't know how strict these, um, kind of, which is the vesicle one, which is the lyposomal. Well, I put protein. So it's sometimes hard to, you know, distinguish [00:11:00] these that the idea is the same.

[00:11:02] Carl Lanore: [00:11:02] So what are these micro RNA regulate? They seem to, uh, obviously seeing them in, in, in the sweat of people who are exercising intensely was the goal, but what did, what did these a micro RNA actually do, uh, in response to exercise, for instance,

[00:11:25] Dr. Sira Karvinen, PhD: [00:11:25] Well in general. Now, if you're, if someone isn't familiar with my prior NACE, they're a small non coding RNA, and they work as a post transcriptional, um, um, regular.

[00:11:40] So basically the idea is that they block the messenger RNA off a protein so that the protein isn't reduced. So it's kind of a negative control. Cycle, but what's, um, fascinating and very difficult as a researcher in micro [00:12:00] RNs is that they're not genes specific. So one of my  can, you know, repressed several my missing Gerard protein.

[00:12:10] So  unsure which one it is, you know, working on

[00:12:16] Carl Lanore: [00:12:16] right now.

[00:12:18] Dr. Sira Karvinen, PhD: [00:12:18] And of course,

[00:12:21] Carl Lanore: [00:12:21] No, no, I was going to say so, so, so this, uh, post transcriptional effect, could this be seen as epigenetic effects where they're, they're, they're making genes do different things as opposed to, you know, could these be considered epigenetic effects?

[00:12:37] Dr. Sira Karvinen, PhD: [00:12:37] Yes. Yes. So

[00:12:41] Carl Lanore: [00:12:41] the presence of this micro RNA may be why exercise seems to have epigenetic effects that are beneficial to humans. Yes,

[00:12:51] Dr. Sira Karvinen, PhD: [00:12:51] it has been studied quite much recently that micro RNA are crucial. First thumb [00:13:00] exercised related adaptations, for instance, uh, angiogenesis or cardiac growth,

[00:13:07] Carl Lanore: [00:13:07] any of them affecting mitochondrial, uh, adaptation or mild mitochondrial size or biogenesis.

[00:13:17] Dr. Sira Karvinen, PhD: [00:13:17] That's really good question. Um, I haven't at least cross in literature of these micro news that I have studied, but there could be

[00:13:30] Carl Lanore: [00:13:30] okay. We'll just describe the exercise, the intensity high intensity exercise that the participants were required to perform.

[00:13:41] Dr. Sira Karvinen, PhD: [00:13:41] Yeah. So we'll want to be able to map. Which is of course all out, besides

[00:13:47] Carl Lanore: [00:13:47] on and on and on an elliptical machine, a stationary bicycle, a treadmill,

[00:13:52] Dr. Sira Karvinen, PhD: [00:13:52] a bike ergometer.

[00:13:53] Yeah.

[00:13:53] Carl Lanore: [00:13:53] Okay. Yeah.

[00:13:55] Dr. Sira Karvinen, PhD: [00:13:55] So they have that first and then according to the results that we [00:14:00] got one day and the lactate levels. So we determined that on Arabic trust, polled and the Arabic trust hold. So the most, you know, on Arabic test, if we're talking about that, What's roughly 5% below that, on this hole that they have.

[00:14:18] But of course they Laclede level variable depending on a person

[00:14:23] Carl Lanore: [00:14:23] th the, the lactate levels were assessed through blood, uh, or through. Okay. So through blood, does lactate come out of the, and in sweat?

[00:14:34] Dr. Sira Karvinen, PhD: [00:14:34] It does. Yes. And there has been some studies of all that, um, sweat Lactaid cause, Oh, Chris, that would be really nice.

[00:14:42] You know, it's the same biomarker that is used in blood, so it would be really convenient to measuring from sweats. Um, and we tried it as well. Believe me, um, There has been a lot of problems in that case, [00:15:00] lactate levels in swept don't really, they don't follow that off serum or plasma or blood blood levels as such.

[00:15:08] Um, probably the problem has to do with just the sweating or the Swift hole kind of increases as the exercise goes on. So lose. The sweat. And then fun thing is also that the sweat glands can produce lactate,

[00:15:28] Carl Lanore: [00:15:28] so, Oh. So they, they could produce lactate on their own. So they're not, they're not just helping the lactate escape the body at that point in time.

[00:15:37] They're actually making it. Yes. So that kind of confuses things. What about the fact that I'm sweating? It evaporates off the skin. I made me a part of, part of the reason we produce sweat is, uh, is to thermal regulate and us. So the sweat actually, um, causes our skin to give off heat, uh, better. So as the, [00:16:00] as you start to just still the sweat, then the theory would be that the concentrations of these things would go up.

[00:16:08] Could that damage the results? The, the, you know, is the reading accurate. Okay,

[00:16:16] Dr. Sira Karvinen, PhD: [00:16:16] that's a good question. And that's also the question of the different, um, ways of harvesting sweats, because you can basically have open air systems, like dripping it. And then, then there's the case that, uh, the water can evaporate.

[00:16:35] So it's kind of more concentrated,

[00:16:38] Carl Lanore: [00:16:38] uh,

[00:16:39] Dr. Sira Karvinen, PhD: [00:16:39] than it would be otherwise. But then again, there's also, you know, if you. Put some kind of plastic on top of the standard to harvest or a filter paper with plastic, then the water kind of evaporates. But then again, the skin temperature probably reaching a loose is higher than it normally would be.

[00:16:59] Oh, there's [00:17:00] always posing in, in that sense. But yeah, it, it would concentrate

[00:17:06] Carl Lanore: [00:17:06] the, whatever it is because I was just thinking of urinalysis when they urine, when they use urine to analyze. And do lab assays with, there is one metabolite in urine that stays constant depending on the concentration of the urine.

[00:17:25] So someone becomes dehydrated. For instance, the urinalysis could be skewed because we say, Oh my goodness, they have so much creatinine in their, in their urine. When in fact they don't, it's just that they're dehydrated. And so there's one metabolite. And I don't remember what it is, but there's one metabolite that they use.

[00:17:43] To standardize. So then they take that metabolite and, and the percentage they find, and then they. Alter all the other metabolites in urine. Could you do that with sweat? Is there something maybe it's sodium that, you know, we know how much sodium is [00:18:00] in sweat and if it, and if we follow that and make that the baseline, then we can extrapolate out all these other concentrations.

[00:18:07] Even if the person's pest perspiration is starting to, uh, is starting to evaporate

[00:18:15] Dr. Sira Karvinen, PhD: [00:18:15] probably. Yeah. Yeah. I haven't really. So a lot of, um, normalizing it's in, in that way, because probably the way we harvested it, wasn't a problem cause he wasn't ever eating. Right. But of course. That could be, you know, one option.

[00:18:32] Carl Lanore: [00:18:32] Right, right. Um, I want to take our first commercial break when we come back. I have more questions we're talking right now with dr. Sera Carvin. Uh, it's an interesting discussion because this opens up the opportunity to use perspiration, uh, to do some sorts of lab analysis. We're talking about really noninvasive, you know, no blood saliva is the closest thing that we have.

[00:18:56] There's also a baseline metabolite in [00:19:00] saliva that they use to adjust a four as well. And this is interesting. We're going to talk more about it on the other side, stay tuned. We'll be right back with more super human radio. We're ripped and we're ready. Welcome back. We're talking with dr. Sierra or Renton or talking about using it sweat as a way to, uh, test for biomarkers.

[00:19:23] Um, So, what do you, what do you hope to, so now that you've identified, uh, this MRN a, which is increased as a result of intense exercise, so you know that you're able to measure it in these circumstances. How, how, how much broader can you go with this testing approach? What else can you test for besides the effects of intense exercise?

[00:19:48] Dr. Sira Karvinen, PhD: [00:19:48] Um, you mean in terms of checking from the.

[00:19:53] Carl Lanore: [00:19:53] Yeah. Yeah, yeah, yeah. So what else can we, what else can you use sweat to, to evaluate instead of saliva or blood or urine? What, [00:20:00] what else can you do with this

[00:20:02] Dr. Sira Karvinen, PhD: [00:20:02] with sweat samples? Well, um, of course our focus is on the exercise intensity. Um, in general, sweat, um, is used for instance, I'm not sure if this was your question, but we'll see how it goes.

[00:20:19] Um, it's used as a diagnostic tool for cystic fibrosis and now there's also emerging, um, field testing, for instance, a mental disorder, some sweats.

[00:20:33] Carl Lanore: [00:20:33] No, you don't, you don't want, you know what that's, that's interesting. And I'll tell you why, because anyone who's ever known someone who's diagnosed with schizophrenia, you could tell that they have a very specific body odor.

[00:20:47] And when my sister developed Parkinson's disease, her body odor changed. And so this is interesting. Um, I had a cousin when I was very young who had cerebral [00:21:00] palsy and he had a very, like, I have an acute sense of smell and I smells can actually trigger memories for me from when I was a kid. And I can tell you that I recognize their body of my sister's body odor changed.

[00:21:15] So that's really interesting that maybe now, along with that scent, there, there are actual biomarkers that can be identified. Huh?

[00:21:25] Dr. Sira Karvinen, PhD: [00:21:25] Yeah, I think it's yeah. Emerging field really. And, you know, in, in cystic fibrosis, in the golden standard already to make her right from the sweat. So, um, hopefully in the future, you know, Parkinson's disease is also one that I have read that there are certain, I can't recall the biomarker, but it has been studied from sweats.

[00:21:47] So it had to be something else than the exercise. It can be search and DCS is.

[00:21:54] Carl Lanore: [00:21:54] So in the case of cystic fibrosis, you said that this is the gold standard. Now [00:22:00] though, the bio marker insisting in sweat that identifies cystic fibrosis, is it not found in serum or saliva or urine?

[00:22:12] Dr. Sira Karvinen, PhD: [00:22:12] That's a good question. Um, they measure it, the  I am from, from sweats that I know from the test, but apparently it's so yeah, easily detectable in the sweat that it's so much higher than with a normal person.

[00:22:31] That's, it's just kind of inevitable that, you know, you either have it or not. But that's a good question. I don't know that much about fibrosis that I could tell how it looks from the block perspective.

[00:22:45] Carl Lanore: [00:22:45] So we know that dogs can be trained to identify certain diseases like cancer. We have dogs now that can sniff a person's body odor and identify whether or not they have cancer, which means to [00:23:00] me that there is it's either exuding from the skin.

[00:23:06] Which means it would probably be in the sweat too. So it could very well be that at some point in time, sweat could be an early detection method for identifying someone with cancer. Now, now we know they have cancer. Now we have to go find out which, which type, you know, it could be like a abroad. Um, a broad approach is to identify specific diseases.

[00:23:29] Then, then you could use other approaches to identify and narrow down. Exactly. Um, what do you think about that?

[00:23:39] Dr. Sira Karvinen, PhD: [00:23:39] I think that's an interesting thought and I think for instance, um, you know, as people leave long war and the chances of getting cancer at some point kind of increases with that. Uh, you know, people could use the noninvasive tests to check, you [00:24:00] know, whether it's kind of alarming or not, for instance, followed by a doctor visit and actual blood testing and stuff.

[00:24:08] So in that sense, it would be very feasible for people also to use for, you know, kind of initial screening. At least.

[00:24:18] Carl Lanore: [00:24:18] I agree. I like, that's what I'm, I'm thinking. Wow. Someday. You could do a sweat test, have it sent into a lab, have it analyzed and have them tell you that they identified this potential for this disease or this potential for this disease.

[00:24:37] That then you would go to a physician and have more specific testing done. Uh, but at least you would have early warning where, and it's so easy to do. You know, if you exercise you and you take a Watman blotter, and you wipe this sweat and absorb it and then put it in a plastic bag and send it to a lab and then they analyze it and they go, Oh my goodness.

[00:24:57] You know, we hate to tell you this, but we found [00:25:00] some of the markers for cancer. You need to go get checked out. Um, you know, and it's funny because you talk about, uh, I just had dr. McKell bloggers cloning on the show, uh, last month. And he has been a pioneer in using, uh, rapamycin as a set of letic. Uh, he, he was an oncologist, uh, and he, and he observed.

[00:25:27] When people with cancer were given Rapp, myosin or Shira Lemus, or any of the rapper logs that he started to notice that they look like their skin got better. They, they, they, they look younger and even in individuals that are given these drugs for, um, for, uh, renal, um, for, for, for kidney, Um, uh, transplants as an anti-rejection drug, you know, they, they literally start to look younger and they don't get sick as often.

[00:25:56] And it was thought that rapid myosin suppressed the immune system. And we [00:26:00] found out that it doesn't. And he came on the show. Uh, he's been on the show numerous times. He came on the show last month, talking about a new study. He just published. And he said, the reason rap, myosin, cures cancer, is it because it reverses the bio biological age of tissue, the older you get, the more likely you are to develop cancer.

[00:26:23] So if you reverse the biological age of tissue and indirect effect is you won't get cancer. And he's convinced that that's how rapid myosin works in curing cancer. And so it would be interesting to find out if since, since all of these things that we're talking about with rapamycin are linked back to the bioaccumulation of senescent cells.

[00:26:50] Senescent cells produce all sorts of inflammatory cytokines and chemokine, and, and cause wholescale inflammation in the body and older, you get. [00:27:00] The more you accumulate, the older you get, the more chronic inflammation you develop and by eradicating senescent cells, whole body inflammation drops. It would be wonderful to see.

[00:27:10] I could test for, uh, yeah. Excessive burden of senescent cells in sweat. That would be, be really interesting because that is an emerging area of interest. There is no way that, you know, The only way to test, to see, uh, uh, senescent cells. So they did a study a couple years ago that we talked about on the show where after one single bout of resistance training exercise, 42% of senescent cells were eradicated from muscle tissue, but how they do that, they had, they had to do punch biopsies.

[00:27:47] Nobody wants to have anybody do a core sample of their muscle. That's painful. But if you had a way to test sweat and identify the presence of senescent cells or, or. [00:28:00] Some unique metabolite of senescent cells that you could say, Oh my goodness, you know, on a scale of zero to a hundred zero being the best and a hundred being, wow, you're in trouble.

[00:28:10] You know, you have a 90, you need to do something to eradicate senescent cells. And there, there are things that can be done today would really be exciting. And that's, that's on the bleeding tip of the spirit today in medicine, it really is.

[00:28:25] Dr. Sira Karvinen, PhD: [00:28:25] Yeah, I agree. And the it's a fascinating idea if we could do that.

[00:28:31] Carl Lanore: [00:28:31] Yeah. Yeah. You'd have to identify some metabolite of Sonesta that is exclusive to the presence of a senescent cell. And then see if you can find that in sweat, that would be so cool. I'd go get that test tomorrow. I would.

[00:28:46] Dr. Sira Karvinen, PhD: [00:28:46] You like biological aging tests from

[00:28:49] Carl Lanore: [00:28:49] sweats. Yeah, exactly. You know, so that everybody's doing biological aging today.

[00:28:54] We have a, we've had a company from the UK, from yeah. From the UK on-call chronometer [00:29:00] and they do 20 million. They identify 20 million points on your DNA. And then they can give you an, an epigenetic readout. This is epigenetic testing that they do so that you can have a biological age versus a chronological age.

[00:29:15] This is very, very emerging right now. If you could do something with sweat that looks for senescent cells, that'll give you biological age. If you have the senescence cell accumulation of an 80 year old person, and you're only 60. So that would be fascinating. There would be a lot of funding. I bet for that kind of research, I really do.

[00:29:36] Um, let's do this. Let's take our last commercial break. And when we come back, I want to ask you some final questions. Uh, this is fascinating stuff. Stay tuned. You're listening to super Yuma radio. We'll be right back where we use oxygen for the power of

[00:29:53] Welcome back. So this study was the first to show that [00:30:00] this micro MRI, uh, M M NRA. Am I saying that right now? Micro RNA. Okay. Sorry. I got a little dyslexic for a second, um, is present and it's present in, um, amounts that make sense too, that it's being produced and, and, and through exercise. So what do you hope to be able to do in the future?

[00:30:26] Are you looking to. Promote this service, uh, for measuring this, or are you just looking to, um, make scientists aware that this is, this is a viable approach to test for certain metabolites?

[00:30:42] Dr. Sira Karvinen, PhD: [00:30:42] Well, I guess the latter that moves sets that's, um, I would like to say here that this is a collaborative project between university of vascular and all.

[00:30:55] And, uh, we are still, um, [00:31:00] we're still waiting for results from all those sides. So, so for our project, we saved only like a very small amount let's and we use their different methods for isolating, uh, the extra care of our bicycle. So what we used was actually the day colon so that we could use the very small, what we use.

[00:31:24] But if we, um, uh, vesicle science, uh, the golden standard ultracentrifugation so we send the book samples that can be used for that. And that can be used them for proteomic profiling or metabolic profiling too. Oh, Oh, we're still okay. Waiting for a more comprehensive analysis of the various samples that we had

[00:31:52] Carl Lanore: [00:31:52] in our study.

[00:31:53] Wow.

[00:31:54] Dr. Sira Karvinen, PhD: [00:31:54] So

[00:31:56] Carl Lanore: [00:31:56] there's some more surprises that you could find. I mean, there's some really [00:32:00] exciting stuff yet to be shown.

[00:32:02] Dr. Sira Karvinen, PhD: [00:32:02] Yes. Yes. So we're hopefully getting a bigger paper out out from that. And, uh, this was just a small kind of outcome where we wanted to show that this was art study set up or the study design and, uh, and then some kind of preliminary results.

[00:32:23] That's you, we never know whether there's something even more interesting coming from the samples. So there might be something that's even more promising than the . There might be some protein there. Some, there might be a metal halides. So, um, I'm not sure what the future holds,

[00:32:42] Carl Lanore: [00:32:42] but it's exciting. It's exciting.

[00:32:43] So, so when do you think you'll get that results back? How is it going to be years from now or.

[00:32:50] Dr. Sira Karvinen, PhD: [00:32:50] Definitely not two years from now, but let's see

[00:32:54] Carl Lanore: [00:32:54] if I can make end up. Yeah. Wow. That's a, it's optimistic. So will [00:33:00] you come back on once that information is available and, and, and give us a presentation again?

[00:33:07] Dr. Sira Karvinen, PhD: [00:33:07] Of course.

[00:33:07] Carl Lanore: [00:33:07] Yeah. Good, good. Yeah, I'm excited. This is really interesting, you know, um, obviously. We measure for, there are certain things that we know that we measure for in sweat already, especially, uh, electrolytes. You know, we know that a lot of the electrolyte science is being driven by the ratios, uh, that we sweat of sodium and potassium and chloride and so on and so forth.

[00:33:32] But that's nothing compared to what you're doing. I mean, what you're doing is really exciting. Like I said, I mean, it's, it could be a gateway to a very simple test that could give you a variety of results and let you know if you're healthy or you need to go see your doctor. Very exciting. Thank you so much for being on the show

[00:33:52] Dr. Sira Karvinen, PhD: [00:33:52] today.

[00:33:54] Thank you. Thank you for having me

[00:33:57] Carl Lanore: [00:33:57] back. Once we have some new research, please I'll make sure [00:34:00] that Alyssa stays in touch with you. Okay.

[00:34:02] Dr. Sira Karvinen, PhD: [00:34:02] Okay. Great. Thank you. I'll be happy to turn you

[00:34:05] Carl Lanore: [00:34:05] to them. Uh, yeah, this is really exciting. I liked this kind of stuff. Anything that, you know, when it comes to, when it comes to lab, uh, analysis of blood or saliva, or, I mean, people don't do enough testing.

[00:34:21] Um, only through testing. Can we determine treatment? Um, too many people, and this is true. And even vitamins and supplements that people take, you know, we have to, uh, like, like vitamin D is a, is, is my favorite one. Vitamin D is one that people take and they really don't know, first of all, if they need it.

[00:34:42] Second of all, if what they're taking is, is, is enough. And, you know, we're lucky here in the United States. I mean, we have, um, lab. Labs that are walking labs. You know, like any lab tests. Now you can walk in there and say, I want this lab work done. You don't have to have a doctor prescribe it. You can pay for it.

[00:35:00] [00:35:00] If you have an a, an, a health savings account, you could pay with your HSA and write that off on your taxes. You know, we're very fortunate when dr. David Zastava introduced saliva testing, uh, probably 16, no, probably 20 years ago. Uh, he was one of my first sponsors back in 2005. Uh, ZRT the labs, they, they, they changed, uh, the way lab work was being done.

[00:35:29] All of a sudden you didn't have to be a physician to get, to get. Lab work done for somebody because it was saliva. They did it in their own home. It was uninvasive, it was convenient. And all of a sudden you had anybody who was a clinician ordering lab work to see where testosterone levels who are, or estrogen levels or, you know, D three levels, a 25 hydroxy.

[00:35:54] So, uh, making it even easier and using something like sweat. Like I said, you get a [00:36:00] Walkman blotter, you wipe your arm, you put it in the bag, you send it off. And they look at all these different things in one shot. That's very, very exciting. So we're going to take a break. And when we come back, YouTube kicked me off this morning for today's show.

[00:36:15] I figured out why I wanted to talk about a study that shows that children who exercise just two times a week for 45 minutes. These are the kids that are like eight, nine, 10, and so on a reduced blood pressure, arterial, stiffness, and a variety of other, uh, pulse, a variety of other things that are harmful.

[00:36:44] And if after, if you live with them, you're going to have cardiac problems later on in life. And so. I guess because the study was done on children, YouTube said, no, no, no, no, no. And they, they, they took me down. I had to rebuild the show and get that out of there, but I [00:37:00] still want to talk about it because parent, okay.

[00:37:03] Um, children are developing high blood pressure and, and vascular problems that we've only ever seen in elderly and adults. And it's really because they don't go out and play anymore. Maybe it's because COVID, you know, that has something to do with it. But children don't go out and play. When I was a kid, I was outside all day long.

[00:37:24] I would come home for dinner. And that was it. I went to school. I played, you know, it's becoming a thing of the past that it's really dangerous because we have children who are developing diseases that we've only ever seen in the elderly. So when we come back. I want to talk about this study, uh, because it's really very important because if we, we already know that a lot of these disorders start in adolescents and then when you're 40 and 50 years old, it's too late to, to change.

[00:37:51] What's already happened. So let's talk about that. When we come back, stay tuned, you're listening and watching superheroes. We feel good.

[00:38:01] [00:38:00] Welcome back. So a study was just published that shows that. A very, very easy exercise, intervention, improved blood pressure parameters, arterial stiffness in children. It was 105 students and they were separated into two groups. One group was given two times a week at 45 minutes of exercise. Um, they all received physical education in their school.

[00:38:30] And so it was done, uh, over the course of 37 weeks. And I'm just going to move real fast with this because the interventional group was given these extra two sessions of 45 minutes a week above and beyond three, uh, PE classes a week at school. What they found out was both exercise performance. Well, what they looked for was exercise performance, uh, peripheral and central blood pressure, pulse pressure.

[00:39:00] [00:38:59] And also augment  pressure, uh, a Nordic pulse as well as arterial stiffness. And what they discovered was in the group, a group that was given the intervention lo and behold, all of these parameters got better. Now. Yeah, this shouldn't surprise anyone. One, because there's been research like this done now for so long unfathomable, but for some reason, schools don't get the message or parents don't get the message.

[00:39:42] Uh, let's face it. Kids want to sit in front of the TV, uh, and play video games. They want to sit in front of the TV and just watch television. They definitely don't want to move, like moving is, is, is no fun. So, so [00:40:00] the reality is that kids just need to be kids. They need to be like, you know what? They got rid of the monkey bars.

[00:40:08] Why because too many kids fell off the monkey bars I fell. I got, I got a line underneath my chin where I fell off the monkey bars and hit my chin. I'm alive. I'm okay. I learned how to climb the monkey bars. You know, monkey bars are like one hand pull ups. I mean, you're climbing that thing. Balancing straddling legs out, hands out.

[00:40:28] I mean, in this, it was amazing when you think about it for dexterity and balance for children, but. Some kid fell and some parent felt like we have to stop climbing monkey bars because their child got hurt. What is happening today? And quite frankly, it's what's happening with YouTube too. What I had in the original, um, title of the show that I was going to cover this, this study, they said that I didn't [00:41:00] meet their, uh, their community criteria and they deleted my show.

[00:41:05] They removed it. All I'm going to do is talk about a study that shows that children who are more active don't end up with high blood pressure, uh, and arterial stiffness. Why is that? Why can't we talk about that? Oh, that's right. Only kind of infants  about science and medicine that, that related to health that you can get has to come from their approved sources.

[00:41:30] Right? What am I saying here? That's so dangerous. Who's going to get hurt. If they say to their child, Hey, I'm good. Sign you up for baseball, football, soccer, tennis, sign your kids up for things that they can do outside of school, because they're going to be healthier. And when they turn 30, 40, and 50, while everybody else is developing heart problems, they're not, we know that we know that the things that [00:42:00] children develop at young ages, if they have high blood pressure, At eight years old.

[00:42:04] I mean, come on, man. They're not going to, I sound like Joe Biden. Come on, man. No, but really they got it. They're gonna, they're gonna get sick at a younger age. Why is that message? So threatening to YouTube, whoever it is that reported that I was going to talk about that they said somebody reported it and after reviewing it, they had a removing.

[00:42:25] Yeah, because I'm going to tell parents about a study. And it's LinkedIn to show you can go and find the actual study PR performed by a legitimate institution that showed that just two sessions a week of 45 minutes of exercise. And what talking about, what are we talking about? Exercise? They're not lifting weights.

[00:42:46] They're probably jogging. You're playing a game. That's it? That's their doing, but that's a bad thing to talk about today. We are so EFT up, you know, I've been saying for years, That [00:43:00] the evolutionary selection pressure today is where you get your information from and who you choose to believe and how you apply.

[00:43:07] What you've learned from those sources to decisions you make will either shorten or lengthen the health span and lifespan of you and your loved ones. That's a fact today. This is a fact, and then you have organizations like YouTube and Facebook and Twitter, trying to limit the information that you get.

[00:43:30] And who is it? That's choosing this, what scientists is working at YouTube that determined that the study I was going to cover would be harmful and dangerous to the public. Who, who, who, who are these PhDs working at these big tech companies, making these decisions. I'd love to find out who their scientists are

[00:43:52] and don't forget, it's not me talking about it. Right because these are legitimate studies. Like I said, it's a LinkedIn today's show. If you go to [00:44:00] my website today, you'll find the actual study. And all this study says is make your kids move more. If you want them not to have high blood pressure and heart disease someday.

[00:44:11] Oh my God. Who am I hurting by saying that this is crazy shit today, man. I'm telling you we are losing freedoms every single day, without even realizing it. It's scary. It really is. And I, and I tell you the truth. So Facebook has bounced me because they claim I made a post that I didn't make and no one will respond to my request to investigate it.

[00:44:38] So I can't stream live to Facebook this morning. I got to wake up, call by YouTube with this. Oh, we're taking your video down. It doesn't meet our criteria. And it didn't take me long to realize. I bet it's the study. I'm going to talk about, about children. A study done by a university. Like, are they, Oh, I get it.

[00:44:57] It's not the science they want to promote. [00:45:00] And I'm thinking I'm going to get away from doing live shows entirely. Uh, for the, for over 13 and a half years, all I depended on was my live stream. That people would tune in on their iPhones and listen to, and then Tom bill, you who I have great respect and admiration for said to me, Carl do video, you got to do video, you've got to do video.

[00:45:24] And I thought, well, I'll do live video. I think I'm going to get away from live video. Those of you who watched the show live, I appreciate your presence. I appreciate your questions. I appreciate your comments, but I'm tired of having to fight with them. Facebook and YouTube. And I think I'm going to go back to being more autonomous.

[00:45:44] I'm going to record the videos. I'm going to live, stream the audio from my server that I own, that they can't block. And then we'll use the video on the website. And I think I'm going to stop becoming dependent [00:46:00] on these organizations. Uh, you know, I don't think anybody's gonna miss the live stream because you'll be able to watch the video or listen to the MP3 at your leisure.

[00:46:11] So I'm tired of it, but yeah, you know what? We didn't have these issues 50 years ago with children because they played outside. Now I get it COVID has been a problem, but that's just the, this year. Childhood obesity is rampant and it didn't just happen in 2020. It's been building for the past 10 years.

[00:46:35] Those children have type two diabetes at 12 and 13 years old. Colon cancer is on the rise in children. Breast cancer is on the rise in young girls. We're talking about 12, 13 years old, and it's not because they're going through puberty. The breast cancer is coming from other things, inflammation. Um, Most of these children are already insulin resistant and [00:47:00] it's up to parents, not the school.

[00:47:03] It's not the school's job to tech your children from illness 30 years from now. It's your job. Okay. Find ways for your children to move more, find ways for your children to exercise. Get them out of the house. Maybe you need to go out with them. Maybe you have a weight problem. Maybe you're insulin resistant.

[00:47:23] Maybe you're on a fast track to getting cancer. Move with them. Save your own life while you save theirs. Walk, just walk around the neighborhood. If you live in a rough neighborhood, join a gym, but we're going to end up having children that die before their parents. It's already starting to happen. It is already, so we're already seeing it, but we're going to have more and more children that die before their parents.

[00:47:51] If we stay on this track and don't, you know, don't be afraid to talk to your kids about it either. Don't be all well, you know, you'll hurt their [00:48:00] feelings and their self esteem. The last generation was all about self esteem and look, look what we have today. All these kids throwing tantrums because things aren't the way they want instead of working to change things.

[00:48:13] So that's it. That was that's the reason I got bounced off YouTube earlier and had to repost the show because they didn't want me to tell you that if your kids don't move more, they're going to end up with heart disease sooner. So don't know. Have your kids look back at you someday and say, mom, dad, why didn't you do something for me?

[00:48:33] Cause they will. Right. Everything gets blamed on the parents. I know everything that happens to the children. It's my fault because of something I did or didn't do when I was raising them. All right. That's it for today, we have a good show tomorrow. Hope you can make it. Um, and I really appreciate the, uh, Oh, I know what I need to say.

[00:48:54] I need people to go ahead and. Um, send me [00:49:00] their SHR story, their testimonial about what it is that they like about SHR, what the show has done for them over the years, because we're not getting the response that I had hoped to. I'm surprised. I thought we had more people submitting, but the reality is that we don't, and I noticed a lot of people out there who've gotten good things out of this show.

[00:49:22] All I'm asking you to do. Is take your phone, whether you do it on video or audio only, and record a brief 32nd video or audio talking about the show, what has it done for you? What hasn't had done for you? Why do you like it? Why don't you like it? And then upload it to SHR network.biz forward slash your story.

[00:49:48] Help me out. I thought we were going to have a lot more people join in on this. Cause I get the, I get great emails from him. People telling me all the time about how much they love the show and how the show has helped them. And thank you so much. I'm asking you to just [00:50:00] put it down in an audio, a video recording and upload it to SHR network.biz/your story.

[00:50:08] So help a brother out. Okay. And, uh, that is it for today. We'll see everybody tomorrow. With more super human radio. And thanks for being here. I don't even have an image to put up here. Okay. So we're going to end the show right now and I'll find an image to put up here. I lost something in the, in the mix here.

[00:50:24] See you tomorrow. [00:51:00]